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KMID : 0357920010350040271
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Korean Journal of Pathology
2001 Volume.35 No. 4 p.271 ~ p.277
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Expression of Cancer-Related Genes in Epstein Barr Virus-Infected Burkitt´s Lymphoma Cell Line Treated with Mitomycin C
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Yeom Bom-Woo
Kim Chul-Hwan
Kim In-Sun
Kim Min-Kyung
Lee Da-Le
Park Seol-Hee
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Abstract
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Background: lnfection of Epstein Barr virus （EBV） into B cells drives the infected cells into the cell cycle and frequently results in lymphoblastoil cells. Mitomycln C inhibits DNA synthesis of eplithelial cells as well as lymphoid cells by cross-linking with DNA. Many of the cancer cells have various pathways for escaping the responsiveness to the negative growth-regulatory effects of mitomycin C and gaining the immortalized property. The auther performed a cell culture of an EBV infected Jijoye lymphoma cell line, and compared the cell cycle and cancer related genes between the mitomycin treated-and non-treated group.
Methods: DNA and RNA were extracted from the Jijoye cells; and EBV nuclear antigen （EBNA）-1, 2and latent membrane protein （LMP） of EBV and p53 and p21 mRNA analyse was performed.
Results: Mitomycin C blocked G2/M phase, however, mitomycin did not affect the expression of EBNA-1, 2 and LMP. Mitomycin C also increased the p21 mRNA expression without p53 mRNA increase.
Conclusions: Mitomycin C induces B cell apoptosis by blocking the G2/M phase and by increasing p21 mRNA independent to p53, which reveals the presence of an alternative pathway of p21 induction by mitomycin C in EBV positive lymphoma cells
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KEYWORD
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Epatein-Barr virus, Mitomycin C, p53, p21, Lymphoma cell
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